Fig. 4

Loss of IFN signaling in MyTrMaSt null mice. a Pathway analysis of the 466 DEGs in WT + PBS versus WT + CBE. Numbers of identified genes out of the total genes associated with each pathway are shown. b Heatmap of representative IFN genes. Red (high, > 1) and blue (low, < 1) in the heat map represent relative gene expression. Some of the DEGs were validated by PCR; WT + CBE versus WT + PBS, fold-change 27.5 ± 2.9 (Irf7), 168.4 ± 27.2 (Irg1); MyTrMaSt + CBE versus MyTrMaSt + PBS, fold-change 1.0 ± 0.1 (Irf7), 2.3 ± 0.2 (Irg1). c Heatmap of the 8 genes upregulated in MyTrMaSt null + CBE versus WT + CBE mice. Some of the DEGs were validated by PCR: MyTrMaSt + CBE versus WT + CBE, fold-change 2.35 ± 0.3 (Serpinf2), 1.93 ± 0.3 (Glycam1), 1.86 ± 0.2 (Apln). d Pathway analysis of the 301 common DEGs. Numbers of identified genes out of the total genes associated with each pathway are shown