Fig. 6

Effect of AD and voluntary physical exercise on morphology of GFAP-positive astrocytes in hippocampi. a Representative reconstructed images of GFAP-positive astrocytes in hippocampi of WT-SED, WT-EXE, 5xFAD-SED, 5xFAD-EXE mice using MicroTrac analysis software. Scale bar 100 μm. Small images display a representative single cell from each experimental group. b Number of GFAP-positive astrocytes per squared mm in hippocampi. c Analysis of GFAP-positive astrocyte morphological parameters such as number of primary branches, total branch length, cell area, cell radius, soma area, cell solidity, and extent. Parameters for each group were normalized to WT-SED values. All data are presented as mean (± SEM). Two-way ANOVA used to measure genotype and exercise effect between WT and 5xFAD mice followed by unpaired t test comparison in case of significant interaction between two factors (genotype*exercise). n = 8/group. «*» Genotype effect, «#» exercise effect: *p < 0.05, ***p < 0.001, #p < 0.05