Fig. 1

Dietary-induced HHcy is associated with increased cortical and hippocampal plaque burden in KI mice. a Representative images of dorsal cortex from KI mice on control diet (KI control) or HHcy diet (KI HHcy). Nuclei were stained with DAPI and are shown in blue; amyloid beta plaques were stained with 6E10 antibody and are shown in white. b Total 6E10 % positive area within cortical and hippocampal ROIs is quantified, showing a statistically significant increase in cortex and hippocampus. The increase in % area positivity for 6E10 corresponded with an increase in the number of plaques per square millimeter in the cortex and hippocampus (c). Levels of soluble Aβ40, Aβ42, and the Aβ42/40 ratios were unchanged in both the hippocampus and cortex (d–i). *p < .05, **p < .01, non-parametric Mann-Whitney U tests