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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Hippocampal interleukin-33 mediates neuroinflammation-induced cognitive impairments

Fig. 1

Effect of hippocampal exogenous IL-33 associated with minocycline pre-treatment on spatial memory and habituation. a Experimental design. Mice were intrahippocampally injected with rmIL-33 or vehicle solution (PBS-BSA 0.1%). The cognitive behavior was tested on day 1 by a first open-field session, followed by a second session on day 2. The short term habituation was analyzed during the first session on day 1. b and d The distance traveled between the first and the last minute in the open-field were compared. c and e The long-term habituation was accessed by comparing the distance traveled between the first session (24 h) and the last session (48 h). Two cohorts have been studied: (b and c) vehicle or rmIL-33 treated without minocycline, (d and e) vehicle or rmIL-33 treated with minocycline. Under our experimental conditions, IL-33 impaired the intersession habituation (c). This effect on spatial memory retrieval was prevented by minocycline administration (e). Values are mean ± SEM, n = 8–15 per group corresponding to a pool of 2 independent experiments. Two-way ANOVA followed by a Sidak post hoc test was used to analyze the distance traveled among groups. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001

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