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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Blocking glycine receptors reduces neuroinflammation and restores neurotransmission in cerebellum through ADAM17-TNFR1-NF-κβ pathway

Fig. 9

Extracellular cGMP normalizes the nuclear content of NF-κB and the levels of glutaminase, TNFα, and mRNA of TNFα in Purkinje cells of hyperammonemic rats. Immunofluorescence was performed using anti-NF-KB (a) and anti-mRNA of TNFα (c) as described in the “Methods” section. Immunohistochemistry was performed as indicated in methods with DAB staining using antibodies against glutaminase (b) and TNFα (d). Representative images are shown. The ratio nuclear/cytoplasmic content of NF-KB (e), the content of glutaminase (f), mRNA of TNFα (g), and TNFα (h) was quantified in Purkinje cells as described in the “Methods” section. Values are mean ± SEM of 6 rats per group. Values significantly different from control rats are indicated by asterisk and from hyperammonemic rats are indicated by “a”. *p < 0.05, ***p < 0.001, ****p < 0.0001; aa p < 0.01, aaa p < 0.001, aaaa p < 0.0001. Data were analyzed using the non-parametric Kruskal-Wallis test followed by Dunnett’s post hoc test (in e) and a one-way analysis of variance (ANOVA) followed by Turkey post hoc (in f-h)

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