Fig. 1

Genotype identification in Hv1^−/− mice. a Tail DNA analyzed by PCR. b Hv1 protein deficiency confirmed by Western blotting. c Expression of Hv1 in microglia assessed by double-immunofluorescence labeling with Hv1 (green) and the microglial marker, IBA-1 (red; scale bar, 50 μm). Hv1 was co-localized with the microglial marker, IBA-1, in wild-type (WT) mice. In contrast, Hv1 immunofluorescent signal was rarely observed in Hv1^−/− (KO) mice