Fig. 2

Spatial and temporal distribution of neuronal pyroptosis and apoptosis after SCI and reduced incidence in Hv1 KO mice. a Representative images of sections showing the pyroptotic marker GSDMD (red), TUNEL (green), and DAPI (blue; scale bar, 500 μm). a The location of the injury core after SCI; b, the area surrounding the injury core after SCI, with apoptosis mainly being located closer to the injury core than pyroptosis (scale bar, 50 μm). b Representative confocal images showing GSDMD (green) and NeuN (red) in sham-operated mice and at 1, 3, and 7 days following SCI (scale bar, 50 μm). c Quantification of the fraction (%) of GSDMD-positive neurons (GSDMD⁺NeuN⁺ cells/NeuN⁺ cells × 100). d Representative images of neurons showing NeuN (red) and TUNEL (green) in sham-operated mice and at 1, 3, and 7 days following SCI (scale bar, 50 μm). e Quantification of the fraction (%) of TUNEL-positive neurons (TUNEL⁺ NeuN⁺ cells/ NeuN⁺ cells × 100). f Western blotting showing cleaved GSDMD. g Quantification of cleaved-GSDMD protein normalized to β-actin from Western blotting (n = 5 for each treatment; ^#p < 0.05 ^##p < 0.01, SCI vs. sham treatment; *p < 0.05 **p < 0.01, KO SCI vs. WT SCI)