Fig. 5

ROS regulates pyroptosis in PC12 cells. a Quantification of LDH release indicating PC12 cell damage and relative ROS levels (DCFH-DA fluorescent intensity normalized to that in control cells). b Representative scanning electron microscopy image showing morphological changes accompanying PC12 cell pyroptosis induced by OGD/R. c Representative confocal images of PC12 cells showing the pyroptotic marker, GSDMD (red), as well as DAPI (blue). d Western-blot analysis of GSDMD. e Quantification of the percentage of GSDMD-positive cells (GSDMD⁺DAPI⁺ cells/DAPI⁺ cells × 100). f Quantification of cleaved GSDMD (from Western blotting) normalized to GAPDH. Data are represented as the mean ± SEM (n = 5 for each treatment; ^##p < 0.01, OGD/R vs. control; *p < 0.05 **p < 0.01, OGD/R + NAC vs. OGD/R)