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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Deficiency of the microglial Hv1 proton channel attenuates neuronal pyroptosis and inhibits inflammatory reaction after spinal cord injury

Fig. 6

ROS modulates apoptosis of PC12 cells. a Representative images of PC12 cells showing TUNEL (green) and DAPI (blue; scale bar, 50 μm). b FACS analysis of PC12 cell apoptosis using Annexin V/PI kit. c Quantification of the percentage of TUNEL-positive cells (TUNEL+DAPI+ cells/DAPI+ cells× 100). d Ratios of apoptotic PC12 cells among different treatment groups (total ratio of apoptosis = Q2 + Q4). Data are represented as the mean ± SEM (n = 5 for each treatment; #p < 0.05 ##p < 0.01, OGD/R vs. control; *p < 0.05 **p < 0.01, OGD/R + NAC vs. OGD/R)

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