Fig. 2From: Splenic sympathetic signaling contributes to acute neutrophil infiltration of the injured spinal cordThoracic SCI promotes an increase in splenic NE levels and alters the expression of NE metabolism-associated genes. a Schematic representation of the impact of a thoracic SCI in the neurotransmitters’ metabolism of postganglionic terminals in the spleen. Created with BioRender.com. b Experimental setup. c–e Catecholamine’s quantification in the spleen 24 hpi by high performance liquid chromatography (HPLC). Quantification data was normalized to spleen weight, and variation relative to respective time-point sham control is shown. Data pooled from two independent experiment. N (1 h) = 6; n (6 h) = 6; n (24 h) = 6. f, g Expression analysis of NE metabolism-associated genes by qPCR in spleens harvested 1, 6, and 24 hpi. Expression analysis was normalized to three housekeeping genes: Gapdh, Hprt, and 18s, and variation relative to respective time-point sham control is shown. N (1 h) = 5; n (6 h) = 5; n (24 h) = 3. Statistical tests: c–g one-way ANOVA; post hoc Tukey’s multiple comparisons test. Results expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001Back to article page