Fig. 5
Splenic denervation led to less neutrophils present at the spinal cord 24 hpi. a Schematic representation of the sympathetic signaling interruption by splenic denervation. Created with BioRender.com. b Experimental setup. c Flow cytometry plots demonstrating gates defining CD45high cells, monocytes (CD45highCD11b+Ly6G-Ly6C+), and neutrophil (CD45highCD11b+Ly6G+Ly6C+) populations present at the spinal cord 24 hpi in SCI vs Dxn + SCI animals. d Frequency of monocytes present at the spinal cord 24 hpi in SCI vs Dxn + SCI animals. e Frequency and concentration of neutrophils at the spinal cord 24 hpi in SCI vs Dxn + SCI animals. N (SCI) = 4; n (Dxn + SCI) = 6. Statistical tests: d, e unpaired t test. Results expressed as mean ± SEM. ***p < 0.001