Fig. 3

Immunohistochemical analysis of activated PBMC. The sections from the hippocampus (a, b, c, and d, respectively), cortex (e, f, g, and h, respectively), thalamus (i, j, k, and l, respectively), cerebellum (m, n, o, and p, respectively), and pons (r, s, t, and u, respectively) of 4.5-month-old WT, Hexa−/−, Neu3−/−, and Hexa−/−Neu3−/− mice were stained with anti-CD45 antibody (red) and DAPI (blue). The histograms represent the quantification of CD45(+) cell number in the hippocampus (v), cortex (w), thalamus (x), cerebellum (y), and pons (z). Scale bar = 50 μm for the hippocampus, cortex, and thalamus; 100 μm for cerebellum and pons. The data are represented as the mean ± SEM. One-way ANOVA was used for statistical analysis (*p < 0.05, **p < 0.025, ***p < 0.01, and ****p < 0.001)