Fig. 5

Immunohistochemical staining for oligodendrocytes. The sections from the cortex (a, b, c, and d, respectively), thalamus (e, f, g, and h, respectively), cerebellum (i, j, k, and l, respectively), and pons (m, n, o, and p, respectively) of 4.5-month-old WT, Hexa−/−, Neu3−/−, and Hexa−/−Neu3−/− mice were stained with anti-CNPase (red) and DAPI (blue). The histograms represent the quantification of oligodendrocytes in the cortex (R), thalamus (S), cerebellum (T), pons (U). Scale bar = 50 μm for cortex and thalamus; 100 μm for cerebellum and pons. The data are represented as the mean ± SEM. One-way ANOVA was used for statistical analysis (*p < 0.05, **p < 0.025, and ****p < 0.001)