Fig. 1
From: Site-specific opening of the blood-brain barrier by extracellular histones
High levels of histones attenuate barrier integrity and TJ staining in mouse brain endothelial cell monolayers. a TEER measurements across confluent monolayers of brain endothelial cells treated with vehicle (saline) or histones (10, 25, 50, and 100 μg/mL). Mean ± S.E.M. Treatments were done in triplicate with four independent biological replicates. b The apparent permeability coefficient (Papp) of brain endothelial cell monolayers to NaFl after 3 h of incubation with either vehicle or histones. Kruskal-Wallis test followed by Dunn’s test for multiple comparisons; n = 4–5 transwells per group; *P = 0.028. Schematic diagram of transwell permeability assay. c, d Immunocytochemical analysis of ZO-1 (c) and claudin-5 (d) in response to treatment of brain endothelial cells with vehicle (saline) or histones at 100 μg/mL. Images are representative of three independent biological replicates. Scale bar = 20 μm