Table 1 Primer sequences used for RT-qPCR
Gene transcript | Accession number | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
Shh | NM_017221.1 | CTGGATTCGACTGGGTCTACTA | GGAAGCAGCCGTCAGATTT |
Spp1 | NM_012881.2 | CACCAAGGACCAACTACAA | TGCCAAACTCAGCCACTT |
Sox8 | NM_001106989.1 | CCCATGGTGAAAGCATGAAAG | TGGGAAAGACCTGTGGTAATG |
Fbln5 | NM_019153.3 | CCTACTCCAATCCCTACTCTACA | TACCCAAAGCGACAGACAAG |
Cd24 | NM_012752.3 | CTTGCCCATTCTGGGATCTAAT | GTTCCCGGGTAGGTTTCTAAAG |
Ngfr | NM_012610.2 | TCTGGCCAAAGAAGAGGATTAC | CATCCTGTGTGTGAGAGAGAAG |
Col8a1 | NM_001107100.1 | CTCTACAGCTGCTGGGAATAC | GTGGTATCTGAGGAGGGATTTG |
Ctsd | NM_134334.2 | CACATCCTTCGACATCCACTAC | TCCACCTTGATACCTCCTAAGT |
Thbs1 | NM_001013062.1 | ACTGAGAGGATGACGACTATG | GTAGGACTGGGTGACTTGTTTC |
Thbs2 | NM_001169138.1 | CCCAGAGGCAGTTTGAGATT | CATCCTCCAGGAAGTTGGTATG |
Icam1 | NM_012967.1 | GTATCCATCCATCCCACAGAAG | CAGTTGTGTCCACTCGATAGTT |
Ccr5 | NM_053960.3 | GCTAGGCAGAGGAGAATGTTAG | TGTCTCCTCCTCCCAGTAAA |
Ccl5 | NM_031116.3 | CAGAGAAGAAGTGGGTTCAAGA | GAGCAAGCAATGACAGGAAAG |
Irf1 | NM_012591.1 | CTCACCAAGAACCAGAGGAAAG | AGATAAGGTGTCAGGGCTAGAA |
Gzmb | NM_138517.3 | AACCAGGAGATGTGTGCTATG | CCTCTTGTAGTGTGTCTGAGTATTT |
Faslg | NM_012908.1 | GGTGCTAATGGAGGAGAAGAAG | TAAATGGTCAGCAACGGTAAGA |
Il10 | NM_012854.2 | AGTGGAGCAGGTGAAGAATG | GAGTGTCACGTAGGCTTCTATG |
Nos2 | NM_012611.3 | TGGAGCGAGTTGTGGATTG | CCTCTTGTCTTTGACCCAGTAG |
Cxcl11 | NM_182952.2 | GTGCCCTGCAAACATTTCTAC | GTGGGAAGCCAGTGTGATTA |
Ifng | NM_138880.2 | CGAATCGCACCTGATCACTAA | TGGATCTGTGGGTTGTTCAC |