Fig. 2
Altered heart rate and augmented hypothermia in mice kept under reduced ambient temperature (Ta) after LPS-induced SIRS response compared with mice kept under neutral Ta irrespective of the genotype (wild-type mice, open boxplots, PI3Kγ-deficient mice (PI3Kγ−/−) filled boxplots, PI3Kγ-kinase-dead mice (PI3KγKD/KD) hatched boxplots). Values are presented as boxplots illustrating medians within boxes from first quartile to the third quartile, whiskers ranging from the 10th to the 90th percentiles (neutral Ta groups: wild-type mice n = 9, PI3Kγ−/− n = 10, PI3KγKD/KD, n = 8; reduced Ta groups: wild-type mice n = 12, PI3Kγ−/− n = 10, PI3KγKD/KD n = 9). * † p < 0.05, asterisk indicates significant difference between baseline and LPS stimulation within each Ta state, dagger sign significant differences versus mice kept under neutral Ta (two-way repeated measures ANOVA, followed by Holm–Sidak test for post hoc multiple comparisons were performed)