Fig. 7
PI3Kγ-dependent suppression of microglial motility and phagocytic activity: a Lipid kinase-dependent reduction of migratory activity induced by brain injury (upper panel) and chemoattractant-stimulated migration of primary microglial cells (lower panel). Reduced ambient temperature as well as reduced temperature of incubation (TInc = 33 °C) led to a decline of microglial motility. b Lipid kinase-independent reduction of phagocytosis was not influenced by ambient/incubation temperatures: reduced number of Iba-1 positive cell with phagocytized zymosan particles in brains obtained from PI3Kγ−/− mice (upper panel) as well as reduced uptake in number of zymosan particles by primary microglial cells obtained from PI3Kγ−/− mice (upper panel). Values are presented as boxplots illustrating medians within boxes from first quartile to the third quartile and whiskers ranging from the 10th to the 90th percentiles (a and b: n = 5 for each group and experimental state. § † p < 0.05, section sign indicates significant differences versus cells derived from wild-type mice kept under same Ta or TInc, dagger sign indicates significant differences versus cells derived from mice kept under normal Ta or TInc at the same experimental state and genotype, two-way ANOVA, followed by Holm–Sidak test for post hoc multiple comparisons, each)