Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Ablation of polyamine catabolic enzymes provokes Purkinje cell damage, neuroinflammation, and severe ataxia

Fig. 6

Tissue and urine polyamine levels in Wt, Sat1-KO, Smox-KO, and Smox/Sat1-dKO mice. Polyamine contents of the cerebellum, cerebrum and kidney of Wt (n = 6), Sat1-KO (n = 4), Smox-KO (n = 4) at 14+ weeks of age, Smox/Sat1-dKO at 8 weeks of age (n = 6) and Smox/Sat1-dKO at 14+ weeks of age (n = 7) were measured in duplicate (t = 2). a The cerebellar spermidine levels decreased significantly in Smox-KO (p = 5.2 × 10−16), 8-week-old Smox/Sat1-dKO (p = 1.82 × 10−19) and 14+-week-old Smox/Sat1-dKO (p = 1.24 × 10−20) compared to Wt mice. The levels of spermine were significantly elevated in the cerebellum of Smox-KO (p = 4.64 × 10−11), 8-week-old Smox/Sat1-dKO (p = 4.87 × 10−20), and 14+-week-old Smox/Sat1-dKO (p = 5.07 × 10−21) compared to Wt mice. Cerebellar polyamine levels of Wt and Sat1-KO mice were not significantly different. b The cerebral spermidine levels decreased significantly in Smox-KO (p = 1.04 × 10−16), pre-ataxic Smox/Sat1-dKO (p = 4.55 × 10−23), and ataxic Smox/Sat1-dKO (p = 1.77 × 10−26) compared to Wt mice. The levels of spermine were significantly elevated in the cerebrum of Smox-KO (p = 2.28 × 10−9), pre-ataxic Smox/Sat1-dKO (p = 9.11 × 10−9), and ataxic Smox/Sat1-dKO (p = 3.09 × 10−16) compared to Wt mice. c Spermidine levels in the kidneys of Smox-KO pre-ataxic Smox/Sat1-dKO and ataxic Smox/Sat1-dKO were similar to Wt mice. The spermidine levels in the kidneys of Smox-KO (p = 5.78 × 10−9), pre-ataxic Smox/Sat1-dKO (p = 2.76 × 10−6), and ataxic Smox/Sat1-dKO (p = 4.46 × 10−9) mice were significantly lower than Wt mice. d The urinary putrescine levels of Smox/Sat1-dKO were significantly lower than Wt mice (p = 0.028), while the urinary levels of other polyamines did not reveal any significant differences. (*) Denotes p < 0.05, (**) denotes p < 0.0001 compared to Wt, (+) denotes p < 0.05 when comparing 14+-week-old Smox/Sat1-dKO mice to 8-week-old Smox/Sat1-dKO and Smox-KO mice

Back to article page