Fig. 8

Increased polyaminated proteins and α-Synuclein expression, polymerization and polyamination are a hallmark of Smox/Sat1-dKO mice. a Comparison of protein polyamination and α-Synuclein expression in the cerebellar and cerebral protein extracts of Wt, 8 weeks old (ataxic) and 14+-week-old (severely ataxic) Smox/Sat1-dKO mice. The lines on the right side of the left panel indicate similar size protein bands that were identified to be differentially polyaminated. b Representative immunofluorescence microscopic images of spermine in the cerebellum of ataxic Smox/Sat1-dKO versus Wt animals indicates the presence of increased staining in cells of the Purkinje layer. The lower panel shows a higher magnification view of upper left panel. c Immunofluorescence microscopy studies examining the expression of α-Synuclein in comparable cerebellar sections of Wt and ataxic Smox/Sat1-dKO animals indicates the presence of increased staining in cells of the Purkinje layer. The lower panel shows a higher magnification view of upper left panel. d α-Synuclein was immunoprecipitated from cerebellar protein extracts of Wt, 8, and 14+-week-old Smox/Sat1-dKO mice, size fractionated and subjected to Western blot analysis using anti-spermine antibodies. The arrows indicate the bands in anti α-Synuclein antibody immunoprecipitates that reacted with anti-spermine antibody. All results are representatives of at least 3 independent experiments. Northern blots contained 25 μg/lane of RNA and western blots contained 50 μg/lane of protein