Fig. 2

HF diet and AD are associated with nonalcoholic fatty liver disease (NAFLD)-related pathology. Liver sections were stained with a hematoxylin and eosin (H&E) or b Sirius red to assess NAFLD pathology. H&E-stained sections were assessed for c steatosis (microvesicular fat), d ballooning (macrovesicular fat), and e inflammation (leukocyte accumulation) using a semi-quantitative scoring system (scored 0–3). f Sirius red-stained sections were assessed for hepatic fibrosis by measuring the percent area positive for the stain. N = 7–12/group for all measures. * = diet effect p < 0.05; *** = diet effect p < 0.001; **** = diet effect p < 0.0001; $$$$ = sex effect p < 0.0001; ^ = AD effect p < 0.05; ^^^^ = AD effect p < 0.0001