Fig. 5

Inflammatory thymic signature in miR-29a/b-1 heterozygous mice in the EAMG model C57BL/6 WT and miR-29a/b-1 HET mice were immunized with CFA/T-AChR or just CFA (for a group of WT mice) at day 0 and boosted at day 30. Mice were sacrificed at day 43. Male mice are represented with grey dots. a The weight of thymuses was normalized to mouse weight. b Representative picture of thymus labeling for cell counting. Thymic sections were stained for TECs with a Keratin 5 antibody (green) and for B cells with a B220 antibody (red). c The number of B cells was counted in 6–8 fields representative of thymic sections and each point corresponds to the mean for each mice. d–u RT-PCR analyses for Cd19, Cxcl13, Ccl21, Cxcr5, Ifnar1, Ifnar2, Ifn-β, Ifn-α2, Il-6, Il-23, Il-17a, Il-21, Ifn-γ, Il-4, Il-10, and Tgf-β1 mRNA expression in mouse thymuses. Data were normalized on GAPDH. p values were assessed by the Mann-Whitney test