Table 3 List of primers used for RT-PCR on mRNAs
From: Decreased expression of miR-29 family associated with autoimmune myasthenia gravis
Gene name | Sens primer | Antisens primer | |
|---|---|---|---|
Human primers | 28S | GGTAGGGACAGTGGGAATCT | CGGGTAAACGGCGGGAGTAA |
CD19 | TACTATGGCACTGGCTGCTG | CACGTTCCCGTACTGGTTCT | |
DICER | TGCTGAAACTGCAACTGACC | CAGGGTCCCAGAACTACCAA | |
GAPDH | CGACCACTTTGTCAAGCTCA | AGGGGTCTACATGGCAACTG | |
IFNAR1 | CCTCCTGTGAGCCTAAGTGC | AAGGGCCTACCCTCAGTGTT | |
Mouse primers | Baff | TTCCATGGCTTCTCAGCTTT | GGAATTGTTGGGCAGTGTTT |
Ccl21 | CCCTGGACCCAAGGCAGT | AGGCTTAGAGTGCTTCCGGG | |
Cd19 | CCCTCACCTTCGAGTTTCTG | TAGGTTCACAGGTCCCAAGG | |
Cxcl13 | TGAGGCTCAGCACAGCAA | ATGGGCTTCCAGAATACCG | |
Cxcr5 | ATGGCCTTAATGTGCCTGTC | CTTCTGGAACTTGCCCTCAG | |
Gapdh | AACTTTGGCATTGTGGAAGG | ACACATTGGGGGTAGGAACA | |
Ifn-β | CCCTATGGAGATGACGGAGA | CTGTCTGCTGGTGGAGTTCA | |
Ifn-α2 | TCTGTGCTTTCCTCGTGATG | TTGAGCCTTCTGGATCTGCT | |
Ifnar1 | CAAGTGTGCCTGGCTTGTTC | AGAGAAGTCCGAGGCCATCT | |
Ifnar2 | ACATGGGTCCTGGCTCAAAG | GGCAGAGAAAGGGTTGCTCT | |
Ifn-γ | CAGCAACAGCAAGGCGAAA | GCTGGATTCCGGCAACAG | |
Il-4 | GGTCTCAACCCCCAGCTAGT | GCCGATGATCTCTCTCAAGTGAT | |
lL-6 | AGTTGCCTTCTTGGGACTGA | TCCACGATTTCCCAGAGAAC | |
Il-10 | GCTCTTACTGACTGGCATGAG | CGCAGCTCTAGGAGCATGTG | |
Il-17a | TTTAACTCCCTTGGCGCAAAA | CTTTCCCTCCGCATTGACAC | |
Il-21 | GGACCCTTGTCTGTCTGGTAG | TGTGGAGCTGATAGAAGTTCAGG | |
Il-23 | CCGTTCCAAGATCCTTCGAA | GACCCGGGCTGCTATGG | |
Tgf-β1 | CAAGGGCTACCATGCCAACT | CCGGGTTGTGTTGGTTGTAGA |