Fig. 2

Heat-killed (HK) Lb is sufficient to ameliorate EAE. a EAE clinical scores and b incidence for C57BL/6J mice orally administered vehicle (PBS), HK Lb, or live Lb daily starting 14 days prior to induction (n = at least 10 mice per group). c Quantification of demyelination by Luxol fast blue (LFB) staining in the spinal cord cross-sections at 30 DPI (n = at least 8 mice per group and at least 6 sections per mouse). d Representative LFB staining of the thoracic spinal cord sections from treated mice. e EAE clinical scores and f incidence for C57BL/6J mice orally administered MRS media or Lb-conditioned media starting 14 days prior to induction (n = at least 11 mice per group). Data are presented as mean ± standard error of the mean (SEM) and are representative of two independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. ns, not significant; AUC one-way ANOVA with Tukey’s multiple comparisons test for a, log-rank test for b and f, one-way ANOVA with Tukey’s multiple comparisons test for c, and AUC Student t test for e