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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response

Fig. 1

Expression levels of PTP1B and proinflammatory genes are upregulated primary astrocytes by TDP-43 overexpression in a, b, astrocytes were transfected with a GFP or TDP-43-GFP expression construct, and after 3 days, FACS of Gfp-transfected live cells was performed. a Successful transfection of Gfp- or TDP-43-Gfp in cells was confirmed by immunoblotting using an anti-TDP-43 or anti-GFP antibody. Tubulin was used for normalization. b Immunoblot analysis of Gfp- or TDP-43-Gfp-transfected cells was performed to detect the expression of PTP1B proteins. Tubulin was used for normalization. Data are presented as the mean ± SD. ***p < 0.001 (Student’s t test). cg TDP-43-Gfp-transfected astrocytes were treated with a PTP1B inhibitor (PTP1Bi, 5 μM) for 1 day, and then real-time PCR was performed. mRNA transcript levels are presented as the mean ± SD. Gapdh was used for normalization. *p < 0.05; ***p < 0.001 (one-way ANOVA). hl Astrocytes were cotransfected with a TDP-43 expression construct and either a control siRNA or a mouse Ptp1b siRNA for 3 days; then, FACS of Gfp-transfected live cells was performed. These cells were allowed to acclimate for 1 day and then were subjected to real-time PCR experiments. mRNA transcript levels are presented as the mean ± SD. Gapdh was used for normalization. *p < 0.05; **p < 0.005; ***p < 0.001 (one-way ANOVA). m Astrocytes were transfected with a Ptp1b-specific siRNA (50 or 100 nM) for 48 h. Immunoblot analysis for the expression level of PTP1B protein. Tubulin was used for normalization. Data are presented as the mean ± SD. ***p < 0.001 (one-way ANOVA)

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