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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response

Fig. 6

The inflammation and neuronal toxicity effects induced by glial TDP-43 are mitigated by Ptp61f downregulation in Drosophila. a, b Levels of Dorsal (Nf-κb) and iNOS mRNA from fly head lysates of control or TDP-43 glial-expressing transgenic flies were analysed by real-time PCR. Gapdh was used for normalization. Data are presented as the mean ± SD. *p < 0.05; **p < 0.005 (one-way ANOVA). c, d Levels of Attacin-C and Diptericin B mRNA from fly head lysates of control or TDP-43-expressing glial transgenic flies were analysed by real-time PCR. Gapdh was used for normalization. Data are presented as the mean ± SD. *p < 0.05; **p < 0.005 (one-way ANOVA). e, f Levels of Tdp-43 and Ptp1b mRNA from fly head lysates of control or TDP-43-expressing glial transgenic flies were analysed by real-time PCR. Gapdh was used for normalization. Data are presented as the mean ± SD. *p < 0.05; ***p < 0.001; N.S. not significant (one-way ANOVA). g Immunostaining of third instar larval NMJs under wild-type (Repo-Gal4/+) and TDP-43-overexpressing (Repo-Gal4/TDP-43) conditions. Labelling of NMJs to determine their morphology in larva was conducted with anti-horseradish peroxidase (green). Scale bars, 50 μm. Data are presented as the mean ± SEM. ***p < 0.001; N.S. not significant (one-way ANOVA). h Survival rate of flies treated with Repo-Gal4/GFP RNAi, Repo,TDP-43/GFP RNAi or Repo,TDP-43/PTP1B RNAi at the indicated time points. Data are presented as the mean ± SEM. i Climbing activity of flies treated with Repo-Gal4/GFP RNAi, Repo, TDP-43/GFP RNAi or Repo,TDP-43/PTP1B RNAi at the indicated time points. Data are presented as the mean ± SEM. *p < 0.05 (one-way ANOVA). j Immunoblot analysis of TDP-43 proteins from head lysates of control or TDP-43 glial transgenic flies. Actin was used for normalization. Data are presented as the mean ± SD. N.S. not significant (Student’s t test). aj Genotypes: GFP is Repo-Gal4/UAS-Egfp RNAiVALIUM20-EGFP.shRNA.4, GFP + PTP1B RNAi is Repo-Gal4/UAS- Ptp61f RNAi HMS00421, TDP-43 is Repo-Gal4/UAS-TDP-43/UAS-Egfp RNAiVALIUM20-EGFP.shRNA.4 and TDP-43+PTP1B RNAi is Repo-Gal4/UAS-TDP-43/UAS-Ptp61f RNAi HMS00421

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