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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Astrocytes from cortex and striatum show differential responses to mitochondrial toxin and BDNF: implications for protection of striatal neurons expressing mutant huntingtin

Fig. 4

BDNF modifies astrocyte cytokine release. Cortical and striatal astrocytes were treated for 24 h with 3NP 15 mM with or without BDNF 50 ng/ml (a) or with LPS 1 μg/ml with or without BDNF 50 ng/ml (b). TNF-α release into the culture supernatant was assessed by ELISA and normalized to viability values obtained with the MTT assay for each experimental group. Data are the mean ± SEM of n = 3 independent experiments. Differences between two groups were analyzed by Student’s t test. ^p < 0.05 vs. 3NP group. Cortical (c) and striatal (d) astrocytes were treated for 24 h with 3NP 15 mM with or without BDNF 50 ng/ml. TGF-β release into the culture supernatant was assessed by ELISA and normalized to viability values obtained by Trypan blue exclusion assay for each experimental group. Data are the mean ± SEM of n = 3 independent experiments. The effect of both factors BDNF and 3NP was analyzed by two-way ANOVA. *p < 0.05 vs. control group ^p < 0.05 vs. 3NP group

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