Fig. 3
MOG35-55-immunized IL6-DIO-KO mice showed an absence of demyelination and CD3 infiltrates, and limited gliosis. a Representative images of LFB staining and CD3 infiltrates (top), astrogliosis and microgliosis (bottom) of the spinal cord from MOG-WT and MOG-IL6-DIO-KO mice. The contrast of the representative images was enhanced. The lack of IL-6 prevented the infiltration of inflammatory cells. The discontinuous line delimits white matter (WM) from gray matter (GM). Scale bar: 100 μm. b From top to bottom: Quantification of demyelination by LFB staining and CD3 immunostaining levels (top) and the total number of GFAP+ and IBA-1+ cells (bottom) in the spinal cord from MOG-WT and MOG-IL6-DIO-KO mice. All results are represented as mean ± SEM; ★p ≤ 0.05 vs. MOG-WT mice