|
CNS region
|
Measure
|
Genotype
|
Repeated saline
|
Repeated morphine
|
Genotype effect
|
Drug effect
|
Genotype x drug
|
|---|
| | | |
mean ± SEM
|
mean ± SEM
|
F1, 56
|
p
|
F1, 56
|
p
|
F1, 56
|
p
|
|---|
|
Striatum
|
Soma area (μm2)
|
Tat(−)
|
51.1 ± 2.16
|
46.7 ± 2.98
|
< 1.0
|
0.49
|
2.9
|
0.09
|
< 1.0
|
0.96
|
|
Tat(+)
|
49.2 ± 2.41
|
45.1 ± 2.00
|
|
Maximum branch length (μm)
|
Tat(−)
|
35.3 ± 3.32
|
32.5 ± 2.59
|
< 1.0
|
0.59
|
< 1.0
|
0.84
|
1.7
|
0.20
|
|
Tat(+)
|
30.7 ± 1.90
|
34.4 ± 2.19
|
|
Critical radius (μm)
|
Tat(−)
|
14.6 ± 1.56
|
13.4 ± 1.35
|
< 1.0
|
0.51
|
< 1.0
|
0.97
|
< 1.0
|
0.63
|
|
Tat(+)
|
14.4 ± 1.76
|
15.6 ± 1.20
|
|
Number of primary process
|
Tat(−)
|
4.8 ± 0.56
|
4.1 ± 0.30
|
2.8
|
0.10
|
< 1.0
|
0.92
|
2.8
|
0.10
|
|
Tat(+)
|
3.5 ± 0.29
|
4.1 ± 0.45
|
|
Process maximum
|
Tat(−)
|
6.8 ± 0.83
|
5.3 ± 0.60
|
< 1.0
|
0.93
|
< 1.0
|
0.70
|
2.3
|
0.13
|
|
Tat(+)
|
5.5 ± 1.00
|
6.4 ± 0.77
|
|
Process total
|
Tat(−)
|
21.5 ± 3.02
|
16.1 ± 2.20
|
< 1.0
|
0.89
|
< 1.0
|
0.70
|
2.1
|
0.16
|
|
Tat(+)
|
16.9 ± 3.49
|
19.9 ± 2.77
|
|
Spinal cord
|
Soma area (μm2)
|
Tat(−)
|
48.5 ± 3.42
|
50.9 ± 3.79
|
4.2
|
0.04
|
3.8
|
0.06
|
1.5
|
0.23
|
|
Tat(+)
|
51.4 ± 2.45
|
62.2 ± 3.73
|
|
Maximum branch length (μm)
|
Tat(−)
|
35.1 ± 2.59
|
36.2 ± 2.71
|
< 1.0
|
0.51
|
< 1.0
|
0.66
|
< 1.0
|
0.98
|
|
Tat(+)
|
33.4 ± 2.32
|
34.6 ± 2.19
|
|
Critical radius (μm)
|
Tat(−)
|
16.7 ± 1.98
|
15.6 ± 1.20
|
< 1.0
|
0.44
|
< 1.0
|
0.98
|
< 1.0
|
0.61
|
|
Tat(+)
|
17.2 ± 2.28
|
18.1 ± 2.04
|
|
Number of primary process
|
Tat(−)
|
2.3 ± 0.28
|
2.7 ± 0.33
|
< 1.0
|
0.81
|
1.5
|
0.23
|
5.0
|
0.03
|
|
Tat(+)
|
3.2 ± 0.47
|
2.0 ± 0.20
|
|
Process maximum
|
Tat(−)
|
3.6 ± 0.31
|
4.6 ± 0.57
|
< 1.0
|
0.81
|
< 1.0
|
0.78
|
5.4
|
0.02
|
|
Tat(+)
|
4.8 ± 0.58
|
3.6 ± 0.27
|
|
Process total
|
Tat(−)
|
11.5 ± 1.56
|
15.7 ± 2.30
|
< 1.0
|
0.58
|
< 1.0
|
0.95
|
5.1
|
0.03
|
|
Tat(+)
|
14.6 ± 1.75
|
10.6 ± 1.22
|
- Sholl analysis of microglial morphology in the striatum and spinal cord of repeated (8-day) saline- or morphine-treated Tat(−) and Tat(+) mice. Data are expressed as the mean ± SEM. The parameters measured by Sholl analysis are indicated in parentheses in the second column. One-way ANOVAs for each CNS region were conducted with genotype and drug as between-subjects factors. F values and p values are presented from ANOVA results. Bolded values denote significant differences at α = 0.05; mean ± SEM, n = 3–4 mice per group/4 sections each
