|
CNS region
|
Lipids
|
Genotype
|
Repeated saline
|
Repeated morphine
|
Genotype effect
|
Drug effect
|
Genotype x drug
|
|---|
|
pg/mg
| |
mean ± SEM
|
mean ± SEM
|
F1, 13
|
p
|
F1, 13
|
p
|
F1, 13
|
p
|
|---|
|
Striatum
|
2-AG
|
Tat(−)
|
11879.7 ± 1270.52
|
15441.7 ± 2440.16
|
< 1.0
|
0.93
|
2.1
|
0.17
|
< 1.0
|
0.52
|
|
Tat(+)
|
13140.9 ± 2086.96
|
14476.7 ± 1229.29
|
|
AEA
|
Tat(−)
|
8.4 ± 0.52
|
9.1 ± 1.87
|
2.3
|
0.15
|
< 1.0
|
0.47
|
2.3
|
0.15
|
|
Tat(+)
|
8.4 ± 1.08
|
6.4 ± 0.33
|
|
NAGly
|
Tat(−)
|
16.0 ± 1.18
|
17.0 ± 1.26
|
< 1.0
|
0.47
|
1.0
|
0.33
|
< 1.0
|
0.85
|
|
Tat(+)
|
16.7 ± 1.55
|
18.10 ± 0.62
|
|
POEA
|
Tat(−)
|
4.9 ± 0.34
|
5.9 ± 0.93
|
1.1
|
0.31
|
< 1.0
|
0.36
|
1.5
|
0.24
|
|
Tat(+)
|
5.0 ± 0.23
|
4.8 ± 0.39
|
|
LEA
|
Tat(−)
|
6.0 ± 0.74
|
7.9 ± 1.71
|
< 1.0
|
0.46
|
< 1.0
|
0.96
|
3.2
|
0.10
|
|
Tat(+)
|
7.1 ± 1.33
|
5.2 ± 0.67
|
|
DEA
|
Tat(−)
|
2.8 ± 0.09
|
3.6 ± 0.91
|
< 1.0
|
0.39
|
1.1
|
0.32
|
1.7
|
0.22
|
|
Tat(+)
|
2.9 ± 0.20
|
2.8 ± 0.16
|
|
DHEa
|
Tat(−)
|
16.1 ± 0.64
|
18.1 ± 4.25
|
< 1.0
|
0.66
|
< 1.0
|
0.58
|
2.9
|
0.11
|
|
Tat(+)
|
18.4 ± 1.99
|
14.3 ± 0.61
|
|
Spinal cord
|
2-AG
|
Tat(−)
|
11168.1 ± 1849.25
|
13770.4 ± 4148.01
|
1.4
|
0.26
|
1.4
|
0.26
|
< 1.0
|
0.90
|
|
Tat(+)
|
9033.6 ± 993.83
|
11126.1 ± 1239.95
|
|
AEA
|
Tat(−)
|
5.8 ± 0.79
|
5.7 ± 2.03
|
< 1.0
|
0.77
|
< 1.0
|
0.60
|
< 1.0
|
0.55
|
|
Tat(+)
|
5.3 ± 0.48
|
7.1 ± 1.98
|
|
NAGly
|
Tat(−)
|
22.8 ± 2.07
|
22.3 ± 4.38
|
< 1.0
|
0.43
|
< 1.0
|
0.74
|
< 1.0
|
0.56
|
|
Tat(+)
|
19.6 ± 1.81
|
21.7 ± 1.19
|
|
POEA
|
Tat(−)
|
8.7 ± 3.12
|
4.9 ± 0.58
|
< 1.0
|
0.83
|
< 1.0
|
0.71
|
< 1.0
|
0.38
|
|
Tat(+)
|
6.7 ± 2.02
|
8.3 ± 3.38
|
|
LEA
|
Tat(−)
|
10.6 ± 4.75
|
5.7 ± 1.40
|
< 1.0
|
0.66
|
< 1.0
|
0.78
|
< 1.0
|
0.52
|
|
Tat(+)
|
9.5 ± 3.85
|
11.4 ± 6.26
|
|
DEA
|
Tat(−)
|
3.9 ± 0.30
|
3.8 ± 1.19
|
< 1.0
|
0.82
|
< 1.0
|
0.37
|
1.1
|
0.32
|
|
Tat(+)
|
3.2 ± 0.42
|
4.3 ± 0.57
|
|
DHEa
|
Tat(−)
|
15.8 ± 0.55
|
18.4 ± 6.49
|
< 1.0
|
0.61
|
1.5
|
0.24
|
< 1.0
|
0.48
|
|
Tat(+)
|
14.7 ± 1.02
|
24.9 ± 7.59
|
- Levels of eCB and non-eCB lipids in the striatum and spinal cord of repeated (8-day) saline- or morphine-treated Tat(−) and Tat(+) mice were not significantly affected by Tat and/or morphine for any of the two CNS regions. Data are expressed as mean ± SEM in pg/mg. Two-way ANOVAs for each CNS region were conducted with genotype and drug as between-subjects factors. F values and p values are presented from ANOVA results. n = 3–5 mice per group. All significant effects are represented in Fig. 7
