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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Chronic alcohol-induced neuroinflammation involves CCR2/5-dependent peripheral macrophage infiltration and microglia alterations

Fig. 1

Alcohol induces infiltration of peripheral macrophages into the CNS. a Alcohol-fed mice received 5% (v/v) alcohol in Lieber-DeCarli liquid diet ad libitum (EtOH) for 6 weeks while pair-fed animals received a calorie-matched liquid diet (PF). b mRNA expression of Ccl2, Ccr2, and Ccr5 was measured from the hippocampus of PF and EtOH mice. c Brain immune cells from wild-type mice fed chronic alcohol (EtOH) or a pair-fed (PF) control diet were isolated and quantified by flow cytometry gating for live, single cells. Microglia (CD11b+ CD45lo) were differentiated from peripheral macrophages (CD11b+ CD45hi) based on surface marker staining and infiltrating macrophages in pair- vs alcohol-fed mice. d Quantification of microglia (CD11b+ CD45lo) and peripheral macrophages (CD11b+ CD45hi) in PF and EtOH mice. Data are mean ± SEM, n = 4–7 mice/group. *p < 0.05 by Student’s t test

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