Fig. 4

HMGB1 induced RAGE expression in microglia. a Representative images of double-staining of RAGE and F4/80 in microglia under normal condition or HMGB1 treatment. Scale bar = 50 μm. b–c Quantification of the numbers of RAGE-positive cells and the IFI/area of RAGE. Note that both the percentage of RAGE-positive cells and IFI of RAGE were significantly increased after HMGB1 treatment. N = 3, ***P < 0.001. IFI, immunofluorescence intensity. d–e Quantification of the protein expression levels of RAGE in control and HMGB1-treated group. β-actin was used as a loading control. Data were expressed as mean ± SEM. N = 3, **P < 0.01