Table 1 PCR primer sequences
Gene | Forward primer(5’-3’) | Reverse primer (5’-3’) |
|---|---|---|
HMGB1 | GCCCATTTTGGGTCACATGG | TGCAGGGTGTGTGGACAAAA |
iNOS | AGAGACGCTTCTGAGGTTCC | CTGCACCAACTCTGCTGTTC |
IL-4 | TCCACGGATGTAACGACAGC | TGGTGTTCCTTGTTGCCGTA |
IL-10 | CCTCTGGATACAGCTGCGAC | GTAGATGCCGGGTGGTTCAA |
IL-12 | ATCATCAAACCGGACCCACC | CAGGAGTCAGGGTACTCCCA |
IL-18 | ACCGCAGTAATACGGAGCAT | TCTGGGATTCGTTGGCTGTT |
CD86 | CGTCAAGACATGTGTAACCTGC | ACCGACTTTTTCCGGTCCTG |
CD206 | TCAACTCTTGGACTCACGGC | CATGATCTGCGACTCCGACA |
TNFα | ACTGAACTTCGGGGTGATCG | TGGTGGTTTGCTACGACGTG |
Arginase1 | ACAAGACAGGGCTACTTTCAGG | ACAAGACAAGGTCAACGCCA |
Ym1 | AGTTTGGATCTGCCCCGTTC | TTAGGAGGGCTTCCACGAGA |
GAPDH | AGTGCCAGCCTCGTCTCATA | GGTAACCAGGCGTCCGATAC |