Fig. 5

Gap19 promotes the degradation of astrocytic Cx43 through the ubiquitin-proteasome pathway after hemin stimulation. a, b WB analysis showed that Gap19 treatment reduced the Cx43 levels induced by hemin stimulation. c qRT-PCR analysis showed that after hemin stimulation, Gap19 treatment had no effect on hemin stimulation-induced Cx43 transcriptional activity. d, e Treatment of cultured astrocytes with MG132 and chloroquine for 12 h. WB analysis showed that MG132 increased the expression of Cx43 after Gap19 treatment (P < 0.05), but there was no significant difference in Cx43 levels between the chloroquine-treated group and the Gap19 + hemin group. f Coimmunoprecipitation with an anti-Cx43 antibody was used to analyze the ubiquitination of Cx43 in astrocytes after hemin stimulation. This figure shows that Gap19 treatment increased the level of Cx43 ubiquitination in astrocytes after hemin stimulation. The bars represent the SEM of the data from 3 samples per group. In d and e, *P < 0.05, **P < 0.01 compared to the hemin group. #P < 0.05, ##P < 0.01 compared with the Gap19 + hemin group. In the rest of the figures, *P < 0.05, **P < 0.01 compared to the control group. #P < 0.05, ##P < 0.01 compared to the hemin group