Fig. 3

Ab-T1 augments uptake of beta amyloid in a Syk-dependent manner and reduces inflammation triggered by IC delivery of soluble TREM2. Ab-T1 increases uptake of labeled oligomeric beta amyloid a in human microglia derived from differentiated human PBMC’s, b murine peritoneal macrophages, and c murine microglia in a Syk dependent manner (R406). The y-axis represents the relative geometric mean (gMFI) measured by flow cytometry. Results are mean of three repeated experiments with duplicate technical sample repetition. d Intracerebral delivery of sTREM2 with Ab-T1 attenuates the neuroinflammatory response evident by delivery of sTREM2 with control IgG (n = 7, two-tailed student’s t test). The y-axis represents the fold-change in expression relative to sham (PBS injected animals)