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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Engagement of TREM2 by a novel monoclonal antibody induces activation of microglia and improves cognitive function in Alzheimer’s disease models

Fig. 4

Effects of Ab-T1 on Syk/Akt phosphorylation and the activation of microglia and macrophages. a Ab-T1 promotes Syk phosphorylation and b Akt phosphorylation. Western blot analysis of phosphorylated Syk (Tyr525/526) or Akt (Ser473) in mouse bone marrow-derived macrophages stimulated with Ab-T1. Ab-T1 upregulates (c) TNFα and (d) IL-1b mRNA and TNFα protein levels in microglia dependent on e Syk or f Akt phosphorylation as the respective inhibitor attenuates consequent Ab-T1-induced activation. Data shown in ELISA assay represent triplicate repeats. g Ab-T1 enhances the uptake of labeled apoptotic neurons by microglia as compared to control IgG. Data shown represent triplicate experimental repeats with triplicate sample repetition

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Journal of Neuroinflammation

ISSN: 1742-2094