Fig. 6

Effects on Ab-T1 on beta amyloid levels and target engagement in 5XFAD mice. a Ab-T1 treatment is associated with a decrease in total brain derived beta amyloid. b Beta amyloid plaques in mice brain sections were confirmed using thioflavin S fluorescent staining. c, d Images taken on microscope were analyzed using ImageJ software for mean number of plaques per mice brain section area and average plaque size (n = 9–10, two-tailed student’s t test, **P = 0.001, *P = 0.032). e Number of diffuse plaques in the cortex of Ab-T1 vs. control-treated 5xFAD mice (two-tailed student’s t test, **P < 0.005). f Ab-T1 induces a reduction of soluble TREM2 in CSF and serum (two-tailed student’s t test, *P = 0.047). g Ab-T1 levels in serum and brain of 5xFAD treated mice. (h; upper panels) Correlation of serum and brain levels of Ab-T1 with cognition tested in MWM (r = 0.46; P < 0.05 and r = 0.5; P < 0.05). (h; lower right) Association between serum and brain levels in the same treated mice (r = 0.89; P < 0.001). (h; lower left) Association of free sTREM2 levels achieved by Ab-T1 treatment, and cognition (r = 0.61; P < 0.05). Data shown in ELISA assays is the mean of triplicate sample repetition