Fig. 6

Axl activation does not affect the mRNA expression of inflammatory cytokines in the spinal cord but increases the production of IL-1β, TNF-α, and IFN-γ in the corpus callosum during acute EAE. RNA was extracted from the spinal cords of IgG isotype control- (n = 8) and α-Axl-treated (n = 7) mice during acute EAE. a mRNA expression of pro-inflammatory cytokines IL-2, IL-17, and IFN-γ; b anti-inflammatory cytokines IL-4, IL-10, and IL-13; c suppressors of cytokine signaling SOCS1 and SOCS3; and d microglia/macrophage marker CD68 during acute EAE. All genes were normalized to HPRT expression. Differences in mRNA expression are shown as 2^−ΔΔCt. e Protein homogenates were prepared from the corpus callosum of IgG isotype control-, α-Axl-, and PBS-treated mice, and protein levels of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ were measured by ELISA (pg/mg total protein) (n = 3–4 per group). Data were analyzed using 2-way ANOVA. *p < 0.05, **p ≤ 0.01, and ***p ≤ 0.001 are considered statistically significant