Fig. 7

Axl antibody activation does not affect the production of inflammatory cytokines or Axl in the spinal cord and brain during chronic EAE. Protein was extracted from whole spinal cords and brains of chronically ill mice. Protein homogenates were prepared from naive, IgG isotype control, and α-Axl-treated mice, and protein levels of the spinal cord a IL-1β, b IL-6, c IL-17, d TNF-α, e IFN-β, and f IFN-γ were measured by ELISA (pg/mg total protein) (n = 8–11). Protein levels of g IL-1β, h IL-6, i IL-17, j TNF-α, k IFN-β, and l IFN-γ in the whole brain tissue were measured by ELISA (pg/mg total protein) (n = 8–11 per group). Protein levels of Axl in the m spinal cord and n brain were measured by ELISA (pg/mg total protein) (n = 6). Data were analyzed using 2-way ANOVA. *p < 0.05 and **p ≤ 0.01 are considered statistically significant