Fig. 8

Characterization of the CNS’s resident astroglial and microglial cell populations by mass cytometry. a EAE clinical score of mice subjected for mass cytometry following treatment with RAM-589.555 (sampling at day 7 after start of RAM-589.555 treatment). n = 6 mice/group; *p < 0.04, **p < 0.01 vs. vehicle-group. Disease incidence is 90%. b Spade diagram visualizing CD45^low CNS residents and CD45^high infiltrating cells. c ViSNE diagrams of brain resident cells visualize the clusters under RAM-589.555 as compared with vehicle-treated and healthy mice. The color code shows the expression levels of CD45, GFAP, and CD11b. Representative diagrams are shown (n = 6 mice/group). The location of the marker positive cells is shown by the ellipse or circle. The data presented are the mean ± SEM according to three independent experimental repeats