Fig. 9

Differential abundance of astroglial and microglial cell populations in RAM-589.555-treated mice. a Spade diagrams of brain resident cells visualize the clusters under the RAM-589.555 as compared with vehicle-treated and healthy mice. The color code shows the expression levels of GFAP⁺ and CD11b⁺ cell populations. Representative diagram are shown (n = 6 mice/group). Dot plots demonstrate the marker labeling on live single cells. GFAP⁺CD45⁻ and CD11b⁺CD45^low populations are contoured by red dashed rectangle. b, c Frequencies of CNS-resident astroglial and microglial cells in RAM-589.555-treated mice as compared with vehicle-treated and healthy mice. The identified astroglia and microglial populations (b and c, respectively) and relative abundance are presented (n = 6 in each group). The data presented are the mean ± SEM according to three independent experimental repeats *p < 0.05; **p < 0.01. Statistical significance was determined by one-way ANOVA with post-hoc Tukey test