Fig. 1
From: Endothelin-1 contributes to the development of virus-induced demyelinating disease
Effect of endothelin-1 on the development of Theiler’s murine encephalomyelitis virus (TMEV)-induced demyelinating disease. a Levels of ET-1 mRNA in the CNS of naïve mice or mice infected with TMEV at 8 dpi. b SJL/J mice (n = 10/group) were intracerebrally infected with a suboptimal dose (1 × 105 PFU) of TMEV and were treated with either PBS or ET-1 (20 μg/kg) at days − 3, 0, 5, 10, 15, 20, and 46. Disease development was evaluated based on the 5-point scale described in the “Materials and methods” section. The difference between the control and ET-1 groups was significant (p < 0.0024) based on the paired two-tailed t test between 37 and 85 dpi. c Relative expression of MCP-1 (CCL2), IP-10 (CXCL-10), KC (CXCL1), TMEV, IFN-γ, and IL-17A mRNA. Real-time PCR was used to assess expression levels. Data are presented as mean ± SEM of three independent experiments. *p < 0.05 and **p < 0.001