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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Endothelin-1 contributes to the development of virus-induced demyelinating disease

Fig. 3

Cytokine levels in the CNS and splenocytes of TMEV-infected ET-1 treated mice. a Levels of cytokines produced by T cells in the CNS of SJL mice at 8 dpi were analyzed by real-time PCR following in vitro re-stimulation with CD4 T cell epitope VP2203-220 and VP425-38 (CD4) and CD8 T cell epitopes VP2121-130 and VP3110-120 (CD8) for 6 h. Levels of IFN-γ and IL-17A T cell cytokine levels are shown. b T cell proliferation and IFN-γ produced after stimulation of CD4+ T cells with UV-TMEV for 4 days were measured using thymidine uptake and ELISA, respectively. Proliferative response of splenic T cells from control and ET-1-treated mice (n = 3) in response to UV-TMEV (5 μg) at 8 days after TMEV infection is shown. Levels of IFN-γ produced by T cells in the CNS following in vitro re-stimulation with either PBS or TMEV antigens are shown. Data are presented as mean ± SEM of three independent experiments. *p < 0.05 and **p < 0.001

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