Fig. 5

Sestrin2 induced microglial M2 polarization by inhibiting mTOR signaling and restoring autophagic flux. a, b Western blot and the quantification for p-mTOR and mTOR in OGD/R treated BV2 cells supplemented with different doses of sestrin2. c, d Western blot and the quantification for p-mTOR and mTOR in OGD treated BV2 cells supplemented with or without MHY-1485. e, f Western blot and the quantification for iNOS and Arginase-1 in OGD treated BV2 cells supplemented with different doses of sestrin2 and MHY-1485. Arg-1 Arginase-1. g, h Western blot and the quantification for p-mTOR/mTOR, LC3II/LC3I, LAMP2/actin in control, OGD, OGD + SESN2, and OGD + SESN2 + MHY-1485 groups. i Immunostaining images for LAMP2 (green) and LC3 (red) of BV2 cells in control, OGD, OGD + SESN2, and OGD + SESN2 + MHY-1485 groups. Scale bar = 25 μm. j, k Quantification of LAMP2 and LC3 relative density in control, OGD, OGD + SESN2, and OGD + SESN2 + MHY-1485 groups. N = 4–6 per group. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001