Fig. 5

Ldlr deletion motivates neuronal pyroptosis in vitro. a, b Dead neurons are detected by PI/Hoechst staining 24 h after reoxygenation, n = 3. Scale bar = 50 μm. c Cell viability of cultured neurons 1 day post-OGD, n = 5–6 in each group. d Immunofluorescence staining for GSDMD and NeuN in the indicated groups, n = 4 in each group. Insets show a higher magnification view. Scale bar = 20 μm. e Western blotting analysis of full-length GSDMD and GSDMD Nterm in neurons 24 h after reoxygenation, n = 5. f, g Extracellular releases of IL-1β and IL-18 in OGD-treated neurons, n = 6–8. All results are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus WT control neurons; ^#P < 0.05 versus WT OGD neurons