Fig. 6

Ldlr knockout amplifies ischemia-induced NLRP3 inflammasome activation. a–d Representative immunofluorescence images of NLRP3, ASC, and caspase-1 were co-stained with NeuN in peri-infarct areas and their quantification 24 h after reperfusion, n = 3–5 in each group. Insets show a higher magnification view. Scale bar = 20 μm. e, f Western blotting and quantitative analysis of NLRP3, ASC, caspase-1, IL-18, and IL-1β expression in ischemic penumbra tissue 24 h after reperfusion, n = 4–6 for each group. Data are indicated as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus WT sham mice; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus WT MCAO mice. Casp 1, Caspase-1