Fig. 7

Absence of LDLR fortifies neuronal inflammasome activation after OGD/R. a Representative immunofluorescence images and quantitative analysis of NLRP3, ASC, and caspase-1 in cultured neurons 24 h after reoxygenation, n = 4. Insets show a higher magnification view. Scale bar = 20 μm. b–f Immunoblotting analysis and quantification of NLRP3, caspase-1, and IL-18 expressions in primary neurons, n = 5 for IL-18 and n = 4 for other proteins. All data are expressed as mean ± SEM. *P < 0.05, ***P < 0.001 versus WT control neurons; ^#P < 0.05 versus WT OGD neurons