Fig. 9

NLRP3 suppression retards Ldlr deletion-induced augment of neuronal pyroptosis. Ldlr^−/− MCAO mice and Ldlr^−/− OGD neurons were treated with NLRP3 inhibitor, CY-09. a, b Expressions of NLRP3, ASC, caspase-1, IL-18, and mature IL-1β in peri-infarct region were analyzed by immunoblots 24 h after ischemia with or without CY-09 injection. c, d Western blotting and quantitative analysis for GSDMD. e, f Immunoblotting analysis and quantitation for NLRP3, ASC, caspase-1, IL-18, and mature IL-1β in Ldlr^−/− neurons 24 h after OGD/R with or without CY-09 treatment. g, h Immunoblots and quantitative analysis for GSDMD in cultured neurons after OGD. n = 4 in each group. Data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 versus Ldlr^−/− sham or Ldlr^−/− control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus Ldlr^−/− MCAO or Ldlr^−/− OGD group