Fig. 4

Resveratrol downregulates TREM2 and the number of microglial cells in NeuP states. a Representative blots and quantification of TREM2 in the ipsilateral SDH at 7 days in SNI rats after intrathecal administration of resveratrol or normal saline. b qPCR analysis of TREM2 expression in the ipsilateral SDH at 7 days in Sham and SNI rats after intrathecal administration of resveratrol or normal saline. c Representative confocal images of ipsilateral SDH showing double staining of TRME2 (red) and Iba-1 (green) at 7 days following SNI in SNI, SNI + NS, and SNI + Res rats. Original magnification, ×20; scale bar, 200 μm. d, e Summarized data for TREM2 intensity (d) and Iba1-positive microglial numbers (e) in the ipsilateral SDH from the three groups shown in (c) (n = 4 rats per group). Data were presented as the mean ± SEM. n = 6 per group. ***p < 0.001 vs. SNI + NS group