Fig. 4

The upregulation of CXCL14 in dorsal horn neurons was involved in mechanical allodynia induced by paclitaxel. a, b Treatment of paclitaxel significantly increased the expression of CXCL14 mRNA and protein on day 5 and day 10 (**P < 0.01 vs the vehicle group, n = 5 per group in real time qPCR, n = 4 per group in western blotting). c The immunohistochemical staining showed that the paclitaxel treatment increased the CXCL14 expression on day 10 compared with vehicle group. Scale bar, 100 μm (n = 3 per group). d, e Intrathecal injection of CXCL14 siRNA significantly attenuated the upregulation of CXCL14 mRNA and protein expression on day 10 following paclitaxel treatment (**P < 0.01 vs the vehicle group, ^##P < 0.01 vs the paclitaxel treatment, n = 5 or 4 per group). f, g Intrathecal injection of CXCL14 siRNA attenuated both mechanical allodynia and thermal hyperalgesia induced by paclitaxel application (**P < 0.01 vs the vehicle group, ^##P < 0.01 vs the paclitaxel treatment, n = 12 per group)