Fig. 5

NFATc2 contributed to the CXCL14 upregulation in dorsal horn following paclitaxel treatment. a Immunofluorescence staining of NFATc2 showed the intensity of NFATc2 (red) was increased in DAPI (blue) positive nuclear region by paclitaxel application on day 10. The histogram analysis of immunofluorescence intensity demonstrated indicates the increased of nucleus translocation of NFATc2 following paclitaxel treatment. Scale bar, 100 μm (**P < 0.01 vs the vehicle group, n = 3 per group). b Double immunostaining showed NFATc2 (red) was colocalized with CXCL14 (green). Left scale bar, 100 μm; right scale bar, 20 μm (n = 3 per group). c Western blotting showed that intrathecal application of FK506 attenuated CXCL14 upregulation induced by paclitaxel (**P < 0.01 vs the vehicle group, ^##P < 0.01 vs the paclitaxel treatment, n = 4 per group). d, e Intrathecal injection of NFATc2 siRNA significantly decreased the increase of CXCL14 mRNA and protein induced by paclitaxel (**P < 0.01 vs the vehicle group, ^##P < 0.01 vs the paclitaxel treatment, n = 4 per group)